BACKGROUND: Teleoperation enables performing tasks in hazardous or inaccessible environments. The relationship between spatial ability and teleoperation performance has been extensively studied; however, relatively few studies have considered examples wherein the specific influence mechanism between the two is examined. This study aims to explore how individuals’ spatial ability affects teleoperation performance.METHODS: Forty subjects completed teleoperated expedition and escape tasks in a virtual unfamiliar environment according to the assigned requirements. After each expedition task, subjects’ mental model about the unfamiliar environment was evaluated. The escape task performance was measured in terms of path length, completion time, and the number of collisions. The impact of spatial ability on escape task performance wherein mental model as a mediator was examined. The Bootstrapping method was used to examine the hypothesis regarding the mediating role of mental model in the influence of spatial ability on teleoperation performance.RESULTS: Subjects with higher spatial ability exhibited significantly better mental models and had fewer collisions. In addition, subjects with better mental models had significantly shorter path lengths and spent marginally less time on escape. In general, the mental model appeared important for path length and completion time, but not collisions.CONCLUSIONS: The combined results of the two tasks preliminarily proved that spatial ability affected path length of the escape task through the mental model after the exploration task. The findings are expected to aid in astronaut selection and teleoperation training for space station missions.Pan D, Liu D, Tian Z, Zhang Y. Performance influence mechanism of individuals’ spatial ability in teleoperation. Aerosp Med Hum Perform. 2022; 93(10):717–724.