INTRODUCTION: High-gravity (G) training is used to educate trainee pilots about anti-G straining maneuvers (AGSM) in an environment similar to that of a real fighter aircraft, and to enhance their G tolerance. The success or failure of high-G training could be multifactorial, but most previous studies have only focused on the effect of pilots’ physical condition.METHODS: A total of 138 male trainee pilots participated in this study. All trainee pilots had received AGSM training from experienced instructors and then underwent centrifuge high-G training. Participants completed questionnaire surveys about body size, lifestyle, self-reported AGSM proficiency, resilience (Connor-Davidson Resilience Scale, CD-RISC), and depression level (Center for Epidemiologic Studies Depression Scale, CES-D).RESULTS: Of the 138 subjects, 100 (72.5%) successfully completed high-G training without experiencing G-induced loss of consciousness (G-LOC) within two trials; these were allocated to the success group. The remaining 38 (27.5%) subjects who completed the training after three or more attempts, or who failed to complete the training at all, were allocated to the failure group. Multivariate analyses revealed that the success of centrifuge training was positively associated with age and self-reported AGSM proficiency, and negatively associated with depression level.DISCUSSION: The success of high-G training was significantly associated with self-reported AGSM proficiency and depression level. Instructors should emphasize the importance of AGSM proficiency and offer practice-based learning to trainee pilots. In addition, they should pay attention to not only trainee pilots’ physical condition, but also their psychological status.Yun C, Oh S, Shin YH. AGSM proficiency and depression are associated with success of high-G training in trainee pilots. Aerosp Med Hum Perform. 2019; 90(7):613–617.